Scientific Skills and Laboratory Techniques

 

  • Molecular & Cell biology Techniques – Mammalian Tissue culture, Transfection/Transduction, Gene silencing via siRNA/CRISPR/shRNA methodologies
  • Protein and RNA-based Techniques – Western blot, SDS-PAGE, RT-PCR, qPCR, ELISA
  • Organelle Fractionation – Nuclear fractionation, Lysosome isolation, Uptake/binding/proteolysis assays
  • Microscopy – Immunofluorescence, Fluorescent and Confocal microscopy, Operetta High-throughput screening
  • In vivo work – Mice breeding and colony maintenance, Cryo-sectioning, Immunohistochemistry, Behavioral analysis, Survival surgeries on mice, Brain slice culturing