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BIO-480 Special topics in Biological Sciences & Bioengineering I – BIOSENSORS

BIO-580/581 Special topics in Biological Sciences & Bioengineering II – BIOSENSORS


Special topics in Biological Sciences & Bioengineering I – BIOSENSORS (BIO580 & BIO480)

Course Objectives :

To provide a broad introduction to biological macromolecules as recognition elements, biosensors and their classification. To give an overall view on basic tools employed in biophysical methods, biomolecular technology and integrating electronic signal transducers. Studies pertaining how engineering and biological approaches can be combined to solve problems in diagnosis of diseases, such as diabetes, cancer or detection of other analytes/biomarkers ? emphasizing integration of biological macromolecules (DNA, enzymes, and antibodies) and methodologies for the development of biosensors. Emphasis will also be given to molecular mechanisms underlying cellular processes and their integration with electronic transducers for biosensor applications.

For more information click HERE

Fall 2010 (Outdated)

Lecture slides- click to download
JHNiazi_Introductory Week -Overview-history-types of biosensors
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Prospective students (Graduate and/or Undergraduate) who wish to take this course are requested to contact me by email: (or) visit me at my office room #2027 between 16:00 and 17:30 (phone extn. 9879).

1. Special topics in Biological Sciences & Bioengineering I – BIOSENSORS (BIO580 200901)



I) History of biosensor development, applications, requirements of biosensors and classification

II) Principles of molecular recognition and transduction signal acquisition: (a) Sources of Biological Recognition elements ? enzymes/proteins, antibody/antigen, receptors, and nucleic acids (b) Design considerations for use of biorecognition elements in biosensors (c) Modeling of reactions for various biosensor applications- electrochemical, optical, piezoelectric, colorimetric, fluorometric and others, (d) Choice of a suitable biological affinity molecule for enhanced sensitivity and specificity,

III) Modification of sensor surfaces and immobilization techniques- (a) Covalent modification and activation of surfaces using surface chemistry ? covalent coupling (b) Covalent attachment of biomolecules on electronic transducer surfaces using homo and hetero bi-functional crosslinkers, corbodiimide mediated chemical coupling, polymerization using soluble (acrylic/glycol) pre-polymers (c) Self Assembled Monolayers (SAM) and adsorption phenomenon (d) Other ways to immobilize biological macromolecules includes, entrapment, encapsulation, and cross-linking on various solid surfaces

IV) Detection methods and Physical Sensors (a) Electrodes/transducers ? electrochemical (amperometric, potentiometric, and conductimetric transductions) (b) optical sensors-colorimetric/ fluorimetric/ luminometric sensors, Surface Plasmon Resonance (SPR) sensors, piezoelectric resonators, cantilevers and other sensors.

V) Design and fabrication of biosensors (a) Biocompatibility of biological elements with electronic transducers? stability, repeatability and compatibility of biomolecules on transducer surfaces (b) Miniaturization-application of nano-materials, nanoparticles, carbon nanotubes (CNTs) and others

VI) Data acquisition, statistical and error analysis (a) Inter and Intra-assays and Coefficient of variation (CV) (b) Signal to noise ratio (c) Normalization/optimization and signal retrieval

VII) Recent developments in commercial biosensors- sensitivity and accuracy for detection of blood constituents (e.g., glucose, urea, creatinine) and disease biomarkers (e.g., diabetes and cancer)
Fall 2010
Special Topics in Biological Sciences and Bioengineering – BIO 580 “BIOSENSORS”
Link – Click here
Download word file (Syllabus)


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