BIO-480 Special topics in Biological Sciences & Bioengineering I – BIOSENSORS
BIO-580/581 Special topics in Biological Sciences & Bioengineering II – BIOSENSORS
Special topics in Biological Sciences & Bioengineering I – BIOSENSORS (BIO580 & BIO480)
Course Objectives :
To provide a broad introduction to biological macromolecules as recognition elements, biosensors and their classification. To give an overall view on basic tools employed in biophysical methods, biomolecular technology and integrating electronic signal transducers. Studies pertaining how engineering and biological approaches can be combined to solve problems in diagnosis of diseases, such as diabetes, cancer or detection of other analytes/biomarkers ? emphasizing integration of biological macromolecules (DNA, enzymes, and antibodies) and methodologies for the development of biosensors. Emphasis will also be given to molecular mechanisms underlying cellular processes and their integration with electronic transducers for biosensor applications.
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Outdated course/syllabus not recommended!
Lecture slides- click to download
JHNiazi_Introductory Week -Overview-history-types of biosensors
Prospective students (Graduate and/or Undergraduate) who wish to take this course are requested to contact me by email: firstname.lastname@example.org (or) visit me at my office room #2027 between 16:00 and 17:30 (phone extn. 9879).
1. Special topics in Biological Sciences & Bioengineering I – BIOSENSORS (BIO580 200901)
I) History of biosensor development, applications, requirements of biosensors and classification
II) Principles of molecular recognition and transduction signal acquisition: (a) Sources of Biological Recognition elements ? enzymes/proteins, antibody/antigen, receptors, and nucleic acids (b) Design considerations for use of biorecognition elements in biosensors (c) Modeling of reactions for various biosensor applications- electrochemical, optical, piezoelectric, colorimetric, fluorometric and others, (d) Choice of a suitable biological affinity molecule for enhanced sensitivity and specificity,
IV) Detection methods and Physical Sensors (a) Electrodes/transducers ? electrochemical (amperometric, potentiometric, and conductimetric transductions) (b) optical sensors-colorimetric/ fluorimetric/ luminometric sensors, Surface Plasmon Resonance (SPR) sensors, piezoelectric resonators, cantilevers and other sensors.
VI) Data acquisition, statistical and error analysis (a) Inter and Intra-assays and Coefficient of variation (CV) (b) Signal to noise ratio (c) Normalization/optimization and signal retrieval