RESEARCH

Future research positions (2016 onwards)

Those who are interested in Postdoctoral position in my lab at Sabanci University Nanotechnology Research & Application Center (SUNUM), Istanbul, please contact me at javed@sabanciuniv.edu. The applicant must include an updated CV and a full list of publications stating any scientific publication on which the applicant wishes to rely. Those who have a strong background in Microbial Biochemistry and a substantial experience in Materials Science and Biophysics are most preferred.

Masters and Ph.D. aspirants can visit here for the regular Admission Requirements for Graduate School of Engineering and Natural Sciences of Sabanci University.

Current research interests

Ref: Qureshi A*, Gurbuz Y and Niazi JH* (2015) Label-free capacitance based aptasensor platform for the detection of HER2/ErbB2 cancer biomarker in serum. Sensors and Actuators B. Chemical 220, 1145-1151.

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Ref: Pandey A, Chouhan RS, Gurbuz Y, Niazi JH* and Qureshi A* (2015) S. cerevisiae whole-cell based capacitive biochip for the detection of toxicity of different forms of carbon nanotubes. Sensors and Actuators B. Chemical, 218, 253-260.

scheme for webpageSchemeRef: Chouhan RS, Qureshi A and Niazi JH* (2015) Determining the fate of fluorescent quantum dots on surface of engineered budding S. cerevisiae cell molecular landscape. Biosensors and Bioelectronics 69, 26-33.

Ref:  Chouhan RS, Qureshi A*, Niazi JH* (2014) Quantum dot conjugated S. cerevisiae as smart nanotoxicity indicators for screening toxicity of nanomaterials. Journal of Materials Chemistry B, 2, 3618-3625.

 

Ref: Niazi JH*, Verma SK, Niazi S and Qureshi A (2015) In vitro HER2 protein-induced affinity dissociation of carbon nanotube-wrapped anti-HER2 aptamers for HER2 protein detection. Analyst, 140, 243-240.

Ref: Qureshi A*, Gurbuz Y and Niazi JH* (2015) Capacitive aptamer-antibody based sandwich assay for the detection of VEGF cancer biomarker in serum. Sensors and Actuators B: Chemical, 209, 645-651.

Ref: Qureshi A,* Pandey A, Chouhan RS, Gurbuz Y, and Niazi JH* (2015) Whole-cell based label-free capacitive biosensor for rapid nanosize-dependent toxicity detection. Biosensors and Bioelectronics, 67, 100-106.

Ref: Chouhan RS, Niazi JH*, Qureshi A* (2014) Chemical toxicity detection using quantum dot encoded E. coli cells. Sensors and Actuators B: Chemical 196, 381-387.

 

ONGOING WORK

[youtube]http://youtu.be/R-yQZspqeRA[/youtube]

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Quantum Dot – E. coli bioconjugates as living cell fluorescent reporters

Ref: Chouhan RS, Niazi JH*Qureshi A** (2013) E. coli-quantum dot bioconjugates as whole-cell fluorescent reporters for probing cellular damagesJournal of Materials Chemistry B,DOI:10.1039/C3TB20338G (Just Accepted).

Scheme for chemical coupling of Quantum Dots on E. coli cell surfaces.

 

 

 

 

 

 

 

 

 

Image Below: Confocal microscopic images of E. coli-QD bioconjugates taken (a) under normal light, (b) after the laser excitation on E. coli-QD bioconjugates and overlayed, (c) loss of fluorescence due to the presence of CNTs after the laser excitation on E. coli-QD bioconjugates and (d) Fluorescence emission spectra of E. coli-QD bioconjugates with or without CNTs incubation showing complete disappearance of fluorescence emission. The fluorescent spectra shown in red and green colors were obtained against PBS as blank. Normalized curves seen in the spectra were obtained when unlabeled E. coli cells were used as control (blank). The inset images show the UV-exposed glass slides spotted with E. coli-QD bioconjugates and incubated by mixing with different ratios of CNTs with respect to QDs (QD:CNT = µM:µg) as indicated in the inset legend.

 

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Fabrication of carbon nanotube decorated magnetic microspheres for storage of energy or separation of biomolecules

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SEM images of naked magnetic microspheres (A), microspheres coated with 0.1 mg CNTs/ 107 microspheres (B, C, D), and microspheres coated with 1 mg CNTs/ 107 microspheres (E, F).

Ref: Unal H, Niazi JH* (2013) Carbon Nanotube Decorated Magnetic Microspheres as an Affinity Matrix for BiomoleculesJournal of Materials Chemistry B, 1, 1894-1902.

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In-vitro selection and modification of synthetic ssDNA/RNA aptamers as affinity ligands for cancer disease biomarkers (proteins) and drugs

Aptamer-Drug Interaction

 

 

 

 

 

 

 

 

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Surface modification for biosensing and assay design using proteins/nucleic acids/whole cells to assess cytotoxicity

Ref: Analyst136, 2726-2734. DOI:10.1039/C1AN15202E [PDF] [Supplementary] (SCI)  This article has been featured in Royal Society of Chemistry blogs- Click here

 

 

 

 

 

 

 

 

 

 

 

 

 

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Multiplexed detection for biosensing

 

 

 

 

 

 

 

 

 

 

 

 

*Qureshi, A., *Niazi, J.H., Kallempudi, S. and Gurbuz, Y. (2010) Label-free capacitive biosensor for sensitive detection of multiple biomarkers using gold interdigitated capacitor arraysBiosensors & Bioelectronics 25, 2318-2323. [PDF] [Supplementary]

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Nanotechnology & Nanotoxicogenomics

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Ref: Niazi, J. H., Sang B. I., Kim, Y.S. and Gu, M.B. (2011) Global gene response in Saccharomyces cerevisiae exposed to silver nanoparticlesApplied Biochemsitry & Biotechnology 164, 1278-1291 DOI: 10.1007/s12010-011-9212-4 [PDF] [Supplementary]

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ssDNA/RNA aptamer directed nano-material/vesicle mediated targeted drug-delivary and/or imaging- ONGOING

[youtube]https://www.youtube.com/watch?v=R-yQZspqeRA&t=6s[/youtube]

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