RESEARCH

Available research positions (immediately)

A Postdoctoral position is available to work in a recently granted TUBITAK project (2019~2021) entitled “Point-of-Care multiplexed detection of Alzheimer’s disease biomarkers using magnetic-quantum dots for early diagnosis of mild cognitive impairment and dementia in elderly people” at Sabanci University Nanotechnology Research Center (SUNUM), Istanbul. Candidates who wish to apply can contact via email to sunum@sabanciuniv.edu (cc to javed@sabanciuniv.edu) with an updated CV and a full list of publications stating any scientific publication on which the applicant wishes to rely. The applicant must have a strong background in chemical synthesis of organic/inorganic nanoparticles (quantum dots) as well as their characterization.

Undergrads (2 part-time) and PhD (1 full-time) positions are available: aspirants can visit here for the regular Admission Requirements for Graduate School of Engineering and Natural Sciences of Sabanci University, or previously registered in any of the universities in Istanbul area.

Current research interests

Ref: Omer Ceylan, Geetesh K. Mishra, Melik Yazici, Anjum Qureshi, Javed H. Niazi, Yasar Gurbuz (2018A Hand-Held Point-of-Care Biosensor Device for Detection of Multiple Cancer and Cardiac Disease Biomarkers using Interdigitated Capacitive Arrays. IEEE Transactions on Biomedical Circuits and Systems, 12(6), 1440-1449.

Ref: Niazi S, Purohit M,  Niazi JH (2018Role of p53 circuitry in tumorigenesis: a brief reviewEuropean Journal of Medicinal Chemistry 158, 7-24.

Ref: Niazi S*, Purohit M, Sonawani A, Niazi JH* (2018Revealing the molecular interactions of aptamers that specifically bind to the extracellular domain of HER2 cancer biomarker protein: An in silico assessmentJournal of Molecular Graphics and Modelling 83, 112-121.

Fig. 6

Ref: Saz M, Siddiq M, Niazi JH, Qureshi A (2018Role of quaternary ammonium compound immobilized metallic graphene oxide in PMMA/PEG membrane for antibacterial, antifouling and selective gas permeability propertiesPolymer Bulletin 1-18.

Scheme 1

Ref: Chouhan RS, Niazi JH, Qureshi A (2018Development of immunoblot assay for carcinoembryonic antigen (CEA) in human serum using portable UV illuminator. Analytical Methods 10, 947-949.

image file: c7ay02929b-s1.tif

Ref: Shaikh T, Pandey A, Sirajuddin, Talpur FN, Kaushik A, Niazi JH* (2017Gold nanoparticles based sensor for in vitro analysis of drug-drug interactions using imipramine and isoniazid drugs: a proof of concept approachSensors and Actuators B. Chemical 252, 1055-1062.

Scheme 1

Ref: Pandey A, Gurbuz Y, Ozguz V, Niazi JH* and Qureshi A* (2017) Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7. Biosensors and Bioelectronics. 91, 225-231.

Ref: Bharadwaj S, Mitchell RJ, Qureshi A, Niazi JH* (2017Toxicity evaluation of e-juice and its soluble aerosols generated by electronic cigarettes using recombinant bioluminescent bacteria responsive to specific cellular damagesBiosensors and Bioelectronics 90, 53-60.

Ref: Chouhan RS, Panday A, Qureshi A*, Ozguz V, Niazi JH* (2016Nanomaterial resistant microorganism mediated reduction of graphene oxideColloids and Surfaces B: Biointerfaces 146, 39-46.

Ref: Chouhan RS, Qureshi A, Yagci B, Gülgün MA, V Ozguz, Niazi JH* (2016) Biotransformation of multi-walled carbon nanotubes mediated by nanomaterial resistant soil bacteria. Chemical Engineering Journal, 298, 1-9.

Ref: Niazi JH*, Pandey A, Gurbuz Y, Ozguz V, Qureshi A (2016Cells-on-chip based transducer platform for probing toxicity of metal nanoparticlesSensors and Actuators B: Chemical, 231, 659–665.

Ref: Qureshi A*, Gurbuz Y and Niazi JH* (2015) Label-free capacitance based aptasensor platform for the detection of HER2/ErbB2 cancer biomarker in serum. Sensors and Actuators B. Chemical 220, 1145-1151.

scheme 222

Ref: Pandey A, Chouhan RS, Gurbuz Y, Niazi JH* and Qureshi A* (2015) S. cerevisiae whole-cell based capacitive biochip for the detection of toxicity of different forms of carbon nanotubes. Sensors and Actuators B. Chemical, 218, 253-260.

scheme for webpageSchemeRef: Chouhan RS, Qureshi A and Niazi JH* (2015) Determining the fate of fluorescent quantum dots on surface of engineered budding S. cerevisiae cell molecular landscape. Biosensors and Bioelectronics 69, 26-33.

Ref:  Chouhan RS, Qureshi A*, Niazi JH* (2014) Quantum dot conjugated S. cerevisiae as smart nanotoxicity indicators for screening toxicity of nanomaterials. Journal of Materials Chemistry B, 2, 3618-3625.

 

Ref: Niazi JH*, Verma SK, Niazi S and Qureshi A (2015) In vitro HER2 protein-induced affinity dissociation of carbon nanotube-wrapped anti-HER2 aptamers for HER2 protein detection. Analyst, 140, 243-240.

Ref: Qureshi A*, Gurbuz Y and Niazi JH* (2015) Capacitive aptamer-antibody based sandwich assay for the detection of VEGF cancer biomarker in serum. Sensors and Actuators B: Chemical, 209, 645-651.

Ref: Qureshi A,* Pandey A, Chouhan RS, Gurbuz Y, and Niazi JH* (2015) Whole-cell based label-free capacitive biosensor for rapid nanosize-dependent toxicity detection. Biosensors and Bioelectronics, 67, 100-106.

Ref: Chouhan RS, Niazi JH*, Qureshi A* (2014) Chemical toxicity detection using quantum dot encoded E. coli cells. Sensors and Actuators B: Chemical 196, 381-387.

 

ONGOING WORK

[youtube]http://youtu.be/R-yQZspqeRA[/youtube]

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Quantum Dot – E. coli bioconjugates as living cell fluorescent reporters

Ref: Chouhan RS, Niazi JH*Qureshi A** (2013) E. coli-quantum dot bioconjugates as whole-cell fluorescent reporters for probing cellular damagesJournal of Materials Chemistry B,DOI:10.1039/C3TB20338G (Just Accepted).

Scheme for chemical coupling of Quantum Dots on E. coli cell surfaces.

 

 

 

 

 

 

 

 

 

Image Below: Confocal microscopic images of E. coli-QD bioconjugates taken (a) under normal light, (b) after the laser excitation on E. coli-QD bioconjugates and overlayed, (c) loss of fluorescence due to the presence of CNTs after the laser excitation on E. coli-QD bioconjugates and (d) Fluorescence emission spectra of E. coli-QD bioconjugates with or without CNTs incubation showing complete disappearance of fluorescence emission. The fluorescent spectra shown in red and green colors were obtained against PBS as blank. Normalized curves seen in the spectra were obtained when unlabeled E. coli cells were used as control (blank). The inset images show the UV-exposed glass slides spotted with E. coli-QD bioconjugates and incubated by mixing with different ratios of CNTs with respect to QDs (QD:CNT = µM:µg) as indicated in the inset legend.

 

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Fabrication of carbon nanotube decorated magnetic microspheres for storage of energy or separation of biomolecules

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SEM images of naked magnetic microspheres (A), microspheres coated with 0.1 mg CNTs/ 107 microspheres (B, C, D), and microspheres coated with 1 mg CNTs/ 107 microspheres (E, F).

Ref: Unal H, Niazi JH* (2013) Carbon Nanotube Decorated Magnetic Microspheres as an Affinity Matrix for BiomoleculesJournal of Materials Chemistry B, 1, 1894-1902.

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In-vitro selection and modification of synthetic ssDNA/RNA aptamers as affinity ligands for cancer disease biomarkers (proteins) and drugs

Aptamer-Drug Interaction

 

 

 

 

 

 

 

 

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Surface modification for biosensing and assay design using proteins/nucleic acids/whole cells to assess cytotoxicity

Ref: Analyst136, 2726-2734. DOI:10.1039/C1AN15202E [PDF] [Supplementary] (SCI)  This article has been featured in Royal Society of Chemistry blogs- Click here

 

 

 

 

 

 

 

 

 

 

 

 

 

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Multiplexed detection for biosensing

 

 

 

 

 

 

 

 

 

 

 

 

*Qureshi, A., *Niazi, J.H., Kallempudi, S. and Gurbuz, Y. (2010) Label-free capacitive biosensor for sensitive detection of multiple biomarkers using gold interdigitated capacitor arraysBiosensors & Bioelectronics 25, 2318-2323. [PDF] [Supplementary]

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Nanotechnology & Nanotoxicogenomics

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Ref: Niazi, J. H., Sang B. I., Kim, Y.S. and Gu, M.B. (2011) Global gene response in Saccharomyces cerevisiae exposed to silver nanoparticlesApplied Biochemsitry & Biotechnology 164, 1278-1291 DOI: 10.1007/s12010-011-9212-4 [PDF] [Supplementary]

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ssDNA/RNA aptamer directed nano-material/vesicle mediated targeted drug-delivary and/or imaging- ONGOING

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